David Functional Annotation Now

DAVID doesn't just count genes. It uses a modified Fisher Exact p-value (EASE score). Look for terms with a p-value < 0.05 after Benjamini correction (FDR). The lower the p-value, the stronger the signal.

Here is your guide to getting the most out of DAVID functional annotation in 2024. Imagine you find 500 genes that are "turned on" during a heart attack. Reading each gene one by one is useless. You will see GAPDH (metabolism), IL6 (inflammation), TNNT2 (contraction), and CASP3 (apoptosis). david functional annotation

Go to [david.ncifcrf.gov] and turn your data into discovery. Have a favorite alternative (Enrichr, g:Profiler, Metascape)? Drop a comment below. But for my money, DAVID is still the gold standard for functional annotation. DAVID doesn't just count genes

This is the most critical step users mess up. You must tell DAVID what the "universe" is. Are you looking at the whole human genome? Or just the 10,000 genes expressed in your specific tissue? Use the whole genome for standard enrichment, but use a tissue-specific background for precision. The lower the p-value, the stronger the signal